DNA replication timing alterations identify common markers between distinct progeroid diseases

Rif1 choreographs DNA replication timing.

Eukaryotic cells duplicate their genome in a predetermined order that appears to reflect a fundamental property of chromatin. Each chromosomal region replicates at a consistent, developmentaland tissue-specific time during the S phase of the cell cycle, and regions that replicate at the same time form distinct patterns in threedimensional nuclear space. Although orderly progression of DNA repli...

Genetic Variation in Human DNA Replication Timing

Genomic DNA replicates in a choreographed temporal order that impacts the distribution of mutations along the genome. We show here that DNA replication timing is shaped by genetic polymorphisms that act in cis upon megabase-scale DNA segments. In genome sequences from proliferating cells, read depth along chromosomes reflected DNA replication activity in those cells. We used this relationship t...

DNA replication timing influences gene expression level

Eukaryotic genomes are replicated in a reproducible temporal order; however, the physiological significance is poorly understood. We compared replication timing in divergent yeast species and identified genomic features with conserved replication times. Histone genes were among the earliest replicating loci in all species. We specifically delayed the replication of HTA1-HTB1 and discovered that...

Dynamic Alterations of Replication Timing in Mammalian Cells

BACKGROUND The eukaryotic genome is divided into distinct replication timing domains, which are activated during S phase in a strictly conserved order. Cellular differentiation can alter replication timing in some loci, but recent experiments yielded conflicting data regarding the relationship between gene expression and replication timing. The genetic and epigenetic determinants of replication...

Simple Sequence Repeat Dna Markers Linked with Genes for Resistance to Major Diseases of Common Bean